Jane Buckner, MD
Title:
Associate Member
Email Address:
Phone Number:
(206) 583-6525
Background
Dr. Buckner received her bachelor’s degree in chemistry from Carleton College, magna cum laude. She attended Johns Hopkins School of Medicine, and after receiving her MD she completed her residency training in Internal Medicine at the University of Minnesota. Dr. Buckner went on to complete a fellowship in Rheumatology at the University of Washington. As a fellow she was honored with the American College of Rheumatology’s Senior Rheumatology Scholar Award. After completing her medical training, Dr. Buckner continued her research training as a postdoctoral fellow in the laboratory of Dr. Gerald Nepom. In 1999, she was the recipient of the ACR Arthritis Investigator Award. Since 1999, Dr. Buckner has been an investigator at the Benaroya Research Institute.
Areas of Research
Dr. Buckner’s laboratory is focused on identifying the underlying mechanisms by which regulation of the adaptive immune response fails or is overcome in the setting of human autoimmunity. The diseases studied in Dr. Buckner’s laboratory include Type 1 diabetes, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosis and relapsing polychondritis. Her laboratory is currently examining the question of how autoreactive T and B cells escape regulation in these diseases and the closely related question of whether the development or function of adaptive Tregs is impaired in autoimmunity. Recently, her group has expanded their focus by utilizing the genetics of autoimmunity to identify the pathways that contribute to these defects. Initial studies have focused on the impact of the PTPN22 1858T variant on lymphocyte function. This work is aided by the availability of human samples for research, which are collected from individuals participating in the Immune Mediated Disease Registry and Repository managed by the Translation Research Program at BRI. This resource provides samples from subjects who are well characterized in terms of clinical characteristics for cellular, biochemical and genetic analysis.
In 2005, Dr. Buckner became the Director of Translational Research at BRI. The goal of this program is to encourage and facilitate research into human immune diseases. Dr. Buckner sees patients with rheumatic diseases at the Virginia Mason Clinic and at the University of Washington as a clinical assistant professor.
Selected Publications
Selected publications:
Buckner J, Kwok WW, Nepom B, Nepom GT. Modulation of HLA-DQ binding properties by differences in class II dimer stability and pH-dependent peptide interactions. J Immunol. 1996; 157:4940-45.
Buckner J and Nepom GT. The role of MHC antigens in autoimmunity. In: Immunologic Aspects of Rheumatic Disease: Cambridge Reviews in Clinical Immunology, J.S.H. Gaston, ed. Cambridge University Press, 1998.
Buckner JH, Wu JJ, Riefe RA, Terato K, Eyre DR. Autoreactivity against matrilin 1 in a patient with relapsing polychondritis. Arthritis Rheum. 2000; 43:939-43.
Gebe JA, Novak EJ, Kwok WW, Farr AG, Nepom GT, Buckner JH. T cell selection and differential activation on structurally related HLA-DR4 ligands. J Immunol. 2001; 167:3250-56.
Buckner JH, Van Landeghen M, Kwok WW, Tsarknaridis L. Identification of type II collagen (261-273) specific T cell clones in a patient with relapsing polychondritis. Arthritis Rheum. 2002; 46:238-44.
Buckner JH, Nepom GT. Genetics of rheumatoid arthritis: is there a scientific explanation for the human leukocyte antigen association? Curr Opin Rheumatol. 2002; 14:254-59.
Buckner JH, Holzer U, Novak EJ, Reijonen H, Kwok WW, Nepom GT. Defining antigen-specific responses with human MHC class II tetramers. J Allergy Clin Immunol. 2002; 110:199-208.
Kwok WW, Ptacek NA, Liu AW, Buckner JH. Use of class II tetramers for identification of CD4+ T cells. J Immunol Methods. 2002; 268:71-81.
Buckner JH and Nepom G. Structure, Function, and Genetics of the HLA Complex. In: Rheumatic Disease in Koopman and Moreland: Arthritis and Allied Conditions 15th edition. Lippincott, Williams & Wilkins, 593-608, 2005.
Holzer U, KwokWW, Nepom GT, Buckner JH. Differential antigen sensitivity and costimulatory requirements in human Th1 and Th2 antigen specific CD4+ cells with similar TCR avidity. J Immunol. 2003; 170:1218-23.
Walker M, Kasprowicz D, Gersuk B, Benard A, Van Landeghen M, Buckner J, Ziegler S. Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4+CD25- T cells. J Clin. Invest. 2003; 112:1437-43.
Kalamasz D, Long SA, Taniguchi R, Buckner JH, Berenson RJ, Bonyhadi M. Optimization of human T-cell expansion ex vivo using magnetic beads conjugated with anti-CD3 and Anti-CD28 antibodies. J Immunother. 2004; 27:405-18.
Buckner JH, Ziegler SF. Regulating the immune system: the induction of regulatory T cells in the periphery. Arthritis Res Ther. 2004; 6:215-22.
Buckner J and Nepom GT. Structure, Function, and Genetics of the Human Leukocyte Antigen Complex in Rheumatic Disease. In: Arthritis and Allied Conditions; Fifteenth Edition, W.J. Koopman and L. W. Moreland, eds. Lippincott Williams and Wilkins, 2005.
Walker MR, Carson BD, Nepom GT, Ziegler SF, Buckner JH. De novo generation of antigen-specific CD4+CD25+ regulatory T cells from human CD4+CD25- cells. Proc Natl Acad Sci U.S.A. 2005; 102:4103-08.
Holzer U, Rieck M, Buckner JH. Lineage and signal strength determine the inhibitory effect of transforming growth factor ?1 (TGF?1) on human antigen-specific Th1 and Th2 memory cells. J Autoimmun. 2006; 26:241-51, Epub 2006.
Lamoureux JL, Buckner JH, David CS, Bradley DS. Mice expressing HLA-DQ6alpha8beta transgenes develop polychondritis spontaneously. Arthritis Res Ther. 2006; 8:R134.
Onengut-Gumuscu S, Buckner JH, Concannon P. A haplotype-based analysis of the PTPN22 locus in type 1 diabetes. Diabetes. 2006; 55:2883-9.
Ziegler SF, Buckner JH. Influence of FOXP3 on CD4+CD25+ regulatory T cells. Expert Rev Clin Immunol (July 2006), 2(4):639-647.
Bollyky PL, Lord JD, Masewicz SA, Evanko SP, Rosenberg C, Buckner JH, Wight TN, Nepom GT. High molecular weight hyaluronan promotes the activation and suppressive effects of CD4+CD25+ T-regs. J Immunol. 2007 Jul 15;179(2):744-7.
Rieck M, Arechiga A, Onegut-Gumuscu S, Greenbaum C, Concannon P, Buckner JH. Genetic variation in PTPN22 corresponds to altered function of T and B lymphocytes. J. Immunology 2007 Oct 1;179(7):4704-10.
Long, SA and Buckner, JH. “Combination of Rapamycin and IL-2 Increases De Novo Induction of Human CD4+CD25+FOXP3+ T Cells.” J of Autoimmunity in press.